[Emergent viruses: SARS-associate coronavirus and H5N1 influenza virus].
Identifieur interne : 000413 ( France/Analysis ); précédent : 000412; suivant : 000414[Emergent viruses: SARS-associate coronavirus and H5N1 influenza virus].
Auteurs : A. Goffard ; L. Lazrek ; C. Schanen ; L. Lobert ; L. Bocket ; D. Dewilde ; D. Hober [France]Source :
- Annales de Biologie Clinique [ 0003-3898 ] ; 1970-01-01.
Abstract
Two viral agents with RNA genome are responsible for emerging illnesses: influenza virus A/H5N1 and Severe Acute Respiratory Syndrome virus (SARS). For the diagnosis of SARS virus infection, an epidemiological investigation is necessary to know whether the patient has been exposed to a risk in a country where the SARS virus is circulating or whether the patient had worked in a laboratory handling SARS virus. The detection of SARS virus is possible in various clinical samples (including urine) by viral culture or RT-PCR. The handling of those samples and RNA extraction must be performed in a BSL3 laboratory. The SARS virus RT-PCR is poorly sensitive, therefore the test should be performed on samples collected consecutively for several days. In front of a suspicion of A/H5N1, similar procedures are recommended. An epidemiologic investigation is necessary to specify whether the patient stayed in a country where A/H5N1 virus was circulating. Clinical samples needed for a specific diagnosis are: nasopharyngeal, throat-swab or fecal samples, cerebrospinal fluid and blood. The presence of A/H5N1 virus is confirmed by viral isolation or RNA detection by RT-PCR. RNA extraction must be performed in a BSL3 laboratory. For diagnosis of A/H5N1 virus infection, RT-PCR test amplifies specifically a fragment of H5 gene (Hemagglutinin). In french laboratories of medical virology, procedures are ready to diagnose the first case of A/H5N1 virus infection and cases of reemerging SARS virus infection.
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Affiliations:
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- to stream Main, to step Curation: 006656
- to stream Main, to step Exploration: 006656
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Hal:hal-02266797Le document en format XML
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<front><div type="abstract" xml:lang="en"> <p>Two viral agents with RNA genome are responsible for emerging illnesses: influenza virus A/H5N1 and Severe Acute Respiratory Syndrome virus (SARS). For the diagnosis of SARS virus infection, an epidemiological investigation is necessary to know whether the patient has been exposed to a risk in a country where the SARS virus is circulating or whether the patient had worked in a laboratory handling SARS virus. The detection of SARS virus is possible in various clinical samples (including urine) by viral culture or RT-PCR. The handling of those samples and RNA extraction must be performed in a BSL3 laboratory. The SARS virus RT-PCR is poorly sensitive, therefore the test should be performed on samples collected consecutively for several days. In front of a suspicion of A/H5N1, similar procedures are recommended. An epidemiologic investigation is necessary to specify whether the patient stayed in a country where A/H5N1 virus was circulating. Clinical samples needed for a specific diagnosis are: nasopharyngeal, throat-swab or fecal samples, cerebrospinal fluid and blood. The presence of A/H5N1 virus is confirmed by viral isolation or RNA detection by RT-PCR. RNA extraction must be performed in a BSL3 laboratory. For diagnosis of A/H5N1 virus infection, RT-PCR test amplifies specifically a fragment of H5 gene (Hemagglutinin). In french laboratories of medical virology, procedures are ready to diagnose the first case of A/H5N1 virus infection and cases of reemerging SARS virus infection.</p>
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<name sortKey="Lobert, L" sort="Lobert, L" uniqKey="Lobert L" first="L" last="Lobert">L. Lobert</name>
<name sortKey="Schanen, C" sort="Schanen, C" uniqKey="Schanen C" first="C" last="Schanen">C. Schanen</name>
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<country name="France"><noRegion><name sortKey="Hober, D" sort="Hober, D" uniqKey="Hober D" first="D." last="Hober">D. Hober</name>
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